GRS1 Antibody

Code CSB-PA339912XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRS1 Polyclonal antibody
Uniprot No.
Target Names
GRS1
Alternative Names
GRS1 antibody; YBR121C antibody; YBR0917 antibody; Glycine--tRNA ligase 1 antibody; mitochondrial antibody; EC 6.1.1.14 antibody; Diadenosine tetraphosphate synthetase antibody; Ap4A synthetase antibody; EC 2.7.7.- antibody; Glycyl-tRNA synthetase 1 antibody; GlyRS 1 antibody; GlyRS1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GRS1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
Gene References into Functions
  1. A phylogenetic analysis further showed that GRS1 and GRS2 are paralogues that arose from a gene duplication event relatively recently, with GRS1 being the predecessor. PMID: 23816885
  2. GRS2 rescued the growth defect of a GRS1 knockout strain when expressed at 37 degrees C, but not at 30 degrees C. These results suggest that GRS2 is actually an inducible gene that may function to rescue the activity of GRS1 under stress conditions. PMID: 22438917
Subcellular Location
[Isoform Cytoplasmic]: Cytoplasm.; [Isoform Mitochondrial]: Mitochondrion matrix.
Protein Families
Class-II aminoacyl-tRNA synthetase family
Database Links

KEGG: sce:YBR121C

STRING: 4932.YBR121C

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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