ileS Antibody

Code CSB-PA512565XA01ENU
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12 / MC4100 / BW2952) ileS Polyclonal antibody
Uniprot No.
Target Names
ileS
Alternative Names
ileS antibody; BWG_0024 antibody; Isoleucine--tRNA ligase antibody; EC 6.1.1.5 antibody; Isoleucyl-tRNA synthetase antibody; IleRS antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12 / MC4100 / BW2952)
Immunogen
Recombinant Escherichia coli (strain K12 / MC4100 / BW2952) ileS protein
Immunogen Species
Escherichia coli (strain K12 / MC4100 / BW2952)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).
Subcellular Location
Cytoplasm.
Protein Families
Class-I aminoacyl-tRNA synthetase family, IleS type 1 subfamily
Database Links

KEGG: ebw:BWG_0024

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