proS Antibody

Code CSB-PA485139XA01EOO
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O8 (strain IAI1) proS Polyclonal antibody
Uniprot No.
Target Names
proS
Alternative Names
proS antibody; ECIAI1_0196 antibody; Proline--tRNA ligase antibody; EC 6.1.1.15 antibody; Prolyl-tRNA synthetase antibody; ProRS antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O8 (strain IAI1)
Immunogen
Recombinant Escherichia coli O8 (strain IAI1) proS protein
Immunogen Species
Escherichia coli O8 (strain IAI1)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.
Subcellular Location
Cytoplasm.
Protein Families
Class-II aminoacyl-tRNA synthetase family, ProS type 1 subfamily
Database Links
CUSABIO guaranteed quality
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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