TFG2 Antibody

Code CSB-PA340454XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFG2 Polyclonal antibody
Uniprot No.
Target Names
TFG2
Alternative Names
TFG2 antibody; YGR005C antibody; Transcription initiation factor IIF subunit beta antibody; TFIIF medium subunit antibody; TFIIF-beta antibody; Transcription factor G 54 kDa subunit antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TFG2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation.
Gene References into Functions
  1. Tfg2 C-terminal domain interacts with double-stranded DNA oligonucleotides. PMID: 22095626
  2. We show that Anc1 is required for growth on galactose as the sole carbon source, and that it is recruited to the UAS of the GAL1 gene after induction. PMID: 15896708
  3. Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of TFIIB near the active center of RNA polymerase II (RNAP II) where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection. PMID: 16147988
  4. Tfg2p is retained in the nucleus through DNA binding in Saccharomyces cerevisiae. PMID: 19366694
Subcellular Location
Nucleus.
Protein Families
TFIIF beta subunit family
Database Links

KEGG: sce:YGR005C

STRING: 4932.YGR005C

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