Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TAA1 Polyclonal antibody

Uniprot No.

Q9S7N2

Target Names

TAA1

Alternative Names

TAA1 antibody; CKRC1 antibody; SAV3 antibody; TIR2 antibody; WEI8 antibody; At1g70560 antibody; F24J13.13 antibody; F5A18.26 antibody; L-tryptophan--pyruvate aminotransferase 1 antibody; EC 2.6.1.27 antibody; EC 2.6.1.99 antibody; Protein CYTOKININ INDUCED ROOT CURLING 1 antibody; Protein SHADE AVOIDANCE 3 antibody; Protein TRANSPORT INHIBITOR RESPONSE 2 antibody; Protein TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 antibody; Protein WEAK ETHYLENE INSENSITIVE 8 antibody; Tryptophan transaminase antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TAA1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

L-tryptophan aminotransferase involved in auxin (IAA) biosynthesis. Can convert L-tryptophan and pyruvate to indole-3-pyruvic acid (IPA) and alanine. Catalyzes the first step in IPA branch of the auxin biosynthetic pathway. Required for auxin production to initiate multiple change in growth in response to environmental and developmental cues. It is also active with phenylalanine, tyrosine, leucine, alanine, methionine and glutamine. Both TAA1 and TAR2 are required for maintaining proper auxin levels in roots, while TAA1, TAR1 and TAR2 are required for proper embryo patterning. Involved in the maintenance of the root stem cell niches and required for shade avoidance.

Gene References into Functions

ARR proteins act as key node that mediate the regulation of auxin biosynthesis by various hormonal, environmental, and developmental signals through transcriptional regulation of the key auxin biosynthesis gene TAA1. PMID: 28931628
In response to Al stress, ethylene signaling locally upregulates TAA1 expression and thus auxin responses in the transition zone (TZ)and results in auxin-regulated root growth inhibition through a number of auxin response factors (ARFs). PMID: 25052716
Arabidopsis ckrc1 mutant encodes tryptophan aminotransferase (TAA1) that is involved in the indole-3-pyruvic acid pathway of indole-3-acetic acid biosynthesis. PMID: 21255165
Biochemical studies revealed that WEI8 encodes a tryptophan aminotransferase, TAA1, in the essential, yet genetically uncharacterized, indole-3-pyruvic acid branch of the auxin synthesis pathway; TAA1 gene was found to correspond to At1g70560. PMID: 18394997

Subcellular Location

Cytoplasm.

Protein Families

Alliinase family

Tissue Specificity

Expressed at the leaf margin and in the vasculature of emerging young leaves. Expressed in the quiescent center and in the vasculature of root tips. Detected in the shoot apical meristem, stems, sepals, stamen filaments, the shoot and root junction, the s

Database Links

KEGG: ath:AT1G70560

STRING: 3702.AT1G70560.1

UniGene: At.27817

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