TAP42 Antibody

Code CSB-PA912211XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TAP42 Polyclonal antibody
Uniprot No.
Target Names
TAP42
Alternative Names
TAP42 antibody; YMR028W antibody; YM9711.18 antibody; YM9973.01C antibody; Type 2A phosphatase-associated protein 42 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TAP42 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.
Gene References into Functions
  1. association of Phosphotyrosyl phosphatase activator with Tap42-phosphatase complexes is rapamycin sensitive PMID: 15689491
  2. Here, we describe the crystal structure of the PP2Ac-interaction domain of Saccharomyces cerevisiae Tap42. The structure reveals an all alpha-helical protein with striking similarity to 14-3-3 and tetratricopeptide repeat (TPR) proteins. PMID: 17616149
  3. These data support a model in which autophagy is negatively regulated by the Tap42-PP2A pathway. PMID: 19223769
Protein Families
IGBP1/TAP42 family
Database Links

KEGG: sce:YMR028W

STRING: 4932.YMR028W

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