tas3 Antibody

Code CSB-PA529725XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tas3 Polyclonal antibody
Uniprot No.
Target Names
tas3
Alternative Names
tas3 antibody; SPBC83.03c antibody; RNA-induced transcriptional silencing complex protein tas3 antibody; RITS protein tas3 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tas3 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex.
Gene References into Functions
  1. The Chp1-Tas3 complex provides a solid and versatile platform to recruit both RNAi-dependent and RNAi-independent gene-silencing pathways for locus-specific regulation of heterochromatin. PMID: 22081013
  2. Tas3 interacts with Chp1 through the C-terminal domain of Chp1, and this interaction is necessary for Tas3 stability. PMID: 15743828
  3. findings suggest a pivotal role for Chp1 and its association with Tas3 for the recruitment of RITS, RDRC, and histone H3 K9 methyltransferase activity to centromeres PMID: 18212052
  4. Tas3 self-association is required for RNA-induced transcriptional silencing (RITS)spreading and efficient heterochromatic gene silencing at centromeric repeat regions. PMID: 19394293
  5. Tas3 subunit of the RNAi effector complex RITS. Specifically localizes to centromeric repeats. PMID: 15607976
  6. Member of the RITS (RNA-induced initiation of transcriptional gene silencing) complex, which is required for RNAi-mediated heterochromatin assembly. [Tas3] PMID: 14704433

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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