tat Antibody

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Code	CSB-PA321860XA01EFZ
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Target Background

Product Details

Full Product Name

Rabbit anti-Equine infectious anemia virus (strain Wyoming) (EIAV) tat Polyclonal antibody

Uniprot No.

P20920

Target Names

tat

Alternative Names

tat antibody; Protein Tat antibody; E-Tat antibody; Transactivating regulatory protein antibody; eTat antibody

Raised in

Rabbit

Species Reactivity

Equine infectious anemia virus (strain Wyoming) (EIAV)

Immunogen

Recombinant Equine infectious anemia virus (strain Wyoming) (EIAV) tat protein

Immunogen Species

Equine infectious anemia virus (strain Wyoming) (EIAV)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to enhance transcription by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a branched hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA). The CDK9 component of P-TEFb hyperphosphorylates the C-terminus of RNA Pol II that becomes stabilized and much more processive (Probable).

Subcellular Location

Host nucleus, host nucleolus.

Protein Families

Lentiviruses Tat family

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tat Antibody

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