mec-12 Antibody

Code CSB-PA309355XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans mec-12 Polyclonal antibody
Uniprot No.
Target Names
mec-12
Alternative Names
mec-12 antibody; tba-3 antibody; C44B11.3 antibody; Tubulin alpha-3 chain antibody; Mechanosensory abnormality protein 12 antibody; MEC-12) [Cleaved into: Detyrosinated tubulin alpha-3 chain] antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans mec-12 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Component of the large-diameter neuronal microtubules which contains 15 protofilaments. Plays a role in mechanosensory transduction (touch sensitivity).
Gene References into Functions
  1. tubulin acetylation and microtubule protofilaments were unaffected in the mec-12(gm379) mutant. We hypothesize that the G416E substitution may alter the association of microtubule-binding proteins with microtubules PMID: 25392990
  2. Acetylation of MEC-12 alpha-tubulin constrains protofilament number in C. elegans touch receptor neurons. PMID: 22658592
  3. Data show that all mec-15 phenotypes can be dominantly suppressed by mutations in mec-7, which encodes a beta-tubulin, and dominantly enhanced by mutations in mec-12, which encodes an alpha-tubulin. PMID: 19652181
Protein Families
Tubulin family
Tissue Specificity
Specifically expressed in neurons.
Database Links

KEGG: cel:CELE_C44B11.3

STRING: 6239.C44B11.3

UniGene: Cel.17899

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