ESK1 Antibody

Code CSB-PA174908XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ESK1 Polyclonal antibody
Uniprot No.
Target Names
ESK1
Alternative Names
ESK1 antibody; TBL29 antibody; At3g55990 antibody; F27K19.170 antibody; Protein ESKIMO 1 antibody; Protein trichome birefringence-like 29 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ESK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues in xylan. Catalyzes the 2-O-acetylation of xylan, followed by nonenzymatic acetyl migration to the O-3 position, resulting in products that are monoacetylated at both O-2 and O-3 positions. Is necessary for the formation of the functional xylem, which is required for water transport to aerial tissues. Acts as negative regulator of cold acclimation. Involved in water economy as well as salt tolerance. Regulated at the transcriptional level by NAC012/SND1.
Gene References into Functions
  1. The esk1-mediated responses counterbalance the defective pattern-triggered immunity (PTI) and Plectosphaerella cucumerina BMM (PcBMM) susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI-mediated resistance is partially compensated by the activation of specific cell-wall-triggered immune responses. PMID: 28792629
  2. a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant. PMID: 25888614
  3. By over-expression of CBF-related genes combining together with suppression of ESK1 gene, the significant increase of plant drought tolerance has been achieved in comparison to single gene manipulation, although the effect is not in an additive way. [ESKMO1] [ESK1] PMID: 25184213
  4. ESK1/TBL29 is involved in the O-acetylation of a wall polysaccharide and that the observed esk1/tbl29 stress phenotypes are a result of a reduction/loss of O-acetyl substituents. [TBL29] PMID: 23340742
  5. Results indicate that ESK1 was localized in the Golgi, the site of xylan biosynthesis, and that ESK1 is a putative xylan acetyltransferase required for 2-O- and 3-O-monoacetylation of xylosyl residues PMID: 23659919
  6. ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components PMID: 21408051
  7. Map-based cloning identified the esk1 locus as At3g55990, ESK1 gene encodes a 57-kDa protein and is a member of a large gene family of DUF231 domain protein which functions as a negative regulator of cold acclimation. PMID: 17316173
  8. Mutations in ESK1 led to lower water content and transpiration rate than wild-type, indicating that ESK1 does not function as a specific cold acclimation gene, but could rather be involved in water homeostasis. PMID: 19054354
  9. ESKIMO1 gene plays a major role in plant response to water shortage as well as cold acclimation and salt tolerance. PMID: 19061521

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Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
PC-esterase family, TBL subfamily
Tissue Specificity
Expressed in roots, young seedlings, leaves, stems, flowers and developing siliques. Specifically expressed in secondary wall-forming cells, xylem and interfascicular fibers.
Database Links

KEGG: ath:AT3G55990

STRING: 3702.AT3G55990.1

UniGene: At.43528

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