tbx2b Antibody

Code CSB-PA800241XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) tbx2b Polyclonal antibody
Uniprot No.
Target Names
tbx2b
Alternative Names
tbx2b antibody; tbx-c antibody; T-box transcription factor TBX2b antibody; T-box protein 2b antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) tbx2b protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor which acts as a transcriptional repressor. May also function as a transcriptional activator. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present in the regulatory region of several genes. Involved in the transcriptional regulation of genes required for mesoderm differentiation. Plays a role in the specification of late notochordal precursor cells and formation of the differentiated notochord. Required for cardiac atrioventricular canal formation.
Gene References into Functions
  1. Bone morphogenetic protein signaling regulates hepatoblast-like cell differentiation into hepatocytes through tbx2b. PMID: 28599080
  2. tbx2a/b mitigate pronephros segmentation downstream of retinoic acid. PMID: 27840199
  3. propose here a rather unique role of Med10 in orchestrating cardiac valve formation by mediating Foxn4 dependent tbx2b transcription, expression of Has2 and subsequently proper development of the cardiac jelly PMID: 27343557
  4. This work establishes a novel link between tbx2b and gdf6a in determining photoreceptor fates. PMID: 24681822
  5. Genetic disruption of the transcription factor Tbx2b eliminates most of the cone subtype maximally sensitive to ultraviolet wavelengths and also perturbs the long-range organization of the cone lattice. PMID: 24465536
  6. Wnt/beta-catenin signaling is both sufficient and required for the induction of BMP4 and Tbx2b expression in the AVC PMID: 21567896
  7. In zebrafish, two tbx2 genes are functionally redundant for regulating chamber development, while each gene is required independently for development of the atrioventricular canal. PMID: 21448936
  8. results uncover an evolutionarily conserved role of Tbx2/3 transcription factors during remodeling of the heart myocardium and highlight the importance of controlling cell proliferation as a driving force of morphogenesis PMID: 17460765
  9. cellular mechanisms regulating neuronal differentiation within the retina are asymmetric about the dorsal/ventral axis, and Tbx2b mediates this process within the dorsal retina PMID: 15755805
  10. Data show that tbx2b transcripts are present during mid-gastrula before its expression is detected by whole-mount in situ hybridization. PMID: 16033799
  11. tbx2b functions to specify the correct number of parapineal cells and to regulate their asymmetric migration. PMID: 18385257
  12. Flh and Tbx2b regulate separate programs of pineal and parapineal development PMID: 18629869
  13. tbx2b in photoreceptor cell precursors promote the UV cone fate by repressing the rod differentiation pathway PMID: 19179291

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the axial mesoderm, notably, in the notochordal precursor cells immediately before formation of the notochord and in the chordoneural hinge of the tail bud, after the notochord is formed. In addition, its expression is detected in the ventral
Database Links

KEGG: dre:30253

STRING: 7955.ENSDARP00000107769

UniGene: Dr.8233

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