Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TCP14 Polyclonal antibody

Uniprot No.

Q93Z00

Target Names

TCP14

Alternative Names

TCP14 antibody; At3g47620 antibody; F1P2.170 antibody; Transcription factor TCP14 antibody; AtTCP14 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TCP14 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor involved the regulation of plant development. Together with TCP15, modulates plant stature by promoting cell division in young internodes. Represses cell proliferation in leaf and floral tissues. Together with TCP15, acts downstream of gibberellin (GA), and the stratification pathways that promote seed germination. Involved in the control of cell proliferation at the root apical meristem (RAM) by regulating the activity of CYCB1-1. Involved in the regulation of seed germination. May regulate the activation of embryonic growth potential during seed germination. Acts together with SPY to promote cytokinin responses that affect leaf shape and trichome development in flowers. Transcription factor involved in the regulation of endoreduplication. Represses endoreduplication by activating the gene expression of the key cell-cycle regulators RBR1 and CYCA2-3. Regulates the expression of the defense gene pathogenesis-related protein 2 (PR2) in antagonism to SRFR1, a negative regulator of effector-triggered immunity. Involved in positive regulation of plant defense. Represses jasmonate (JA) response to promote disease resistance. Regulates the plant immune system by transcriptionally repressing a subset of JA-responsive genes.

Gene References into Functions

TCP14 regulates the plant immune system by transcriptionally repressing a subset of the jasmonic acid (JA) hormone signaling outputs. The Pseudomonas syringae type III effector, HopBB1, interacts with TCP14 and targets it to the SCF(COI1) degradation complex by connecting it to the JA signaling repressor JAZ3. HopBB1 de-represses the TCP14-regulated subset of JA response genes and promotes pathogen virulence. PMID: 28132837
TCP14 and TCP15 act downstream of the gibberellins and promote seed germination. PMID: 25655823
DOF6 negatively affects seed germination and opposes TCP14 function in the regulation of a specific set of ABA-related genes. PMID: 22155632
Together, the two genes, TCP14 and TCP15, influence plant stature by promoting cell division in young internodes. PMID: 21668538

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in the vascular tissues of the embryo. Expressed in young proliferating tissues.

Database Links

KEGG: ath:AT3G47620

STRING: 3702.AT3G47620.1

UniGene: At.21251

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TCP14 Antibody

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