tdcB Antibody

Code CSB-PA313334XA01EOD
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 tdcB Polyclonal antibody
Uniprot No.
Target Names
tdcB
Alternative Names
tdcB antibody; Z4469 antibody; ECs3997 antibody; L-threonine dehydratase catabolic TdcB antibody; EC 4.3.1.19 antibody; L-serine dehydratase antibody; EC 4.3.1.17 antibody; Threonine deaminase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7
Immunogen
Recombinant Escherichia coli O157:H7 tdcB protein
Immunogen Species
Escherichia coli O157:H7
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA. TdcB also dehydrates serine to yield pyruvate via analogous enamine/imine intermediates.
Protein Families
Serine/threonine dehydratase family
Database Links

KEGG: ece:Z4469

STRING: 155864.Z4469

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