Nu1 Antibody

Code CSB-PA366025XA01FSF
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Product Details

Full Product Name
Rabbit anti-Escherichia phage lambda (Bacteriophage lambda) Nu1 Polyclonal antibody
Uniprot No.
Target Names
Nu1
Alternative Names
Nu1 antibody; lambdap01 antibody; Terminase small subunit antibody; EC 3.6.4.- antibody; DNA-packaging protein Nu1 antibody; Gene product Nu1 antibody; gpNu1 antibody
Raised in
Rabbit
Species Reactivity
Escherichia phage lambda (Bacteriophage lambda)
Immunogen
Recombinant Escherichia phage lambda (Bacteriophage lambda) Nu1 protein
Immunogen Species
Escherichia phage lambda (Bacteriophage lambda)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The small subunit is responsible for the binding to multiple recognition elements within the packaging initiation site cos. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (binding to packaging sequence cos), and a large terminase subunit possessing endonucleolytic, ATPase and helicase activities (DNA maturation and packaging). The terminase binds, cooperatively with the host factor IHFA/IHFB, to the cos site at the junction of adjacent viral genomes in the concatemeric DNA. The endonuclease and helicase activities of the large subunit cleave the viral DNA generating 5'overhangs of 12 bp in length. The terminase remains bound to the left end of the genome to be packaged, forming a stable DNA-terminase complex. In a reaction facilitated by the viral assembly catalyst gpFI, the DNA-terminase complex binds to the portal of the procapsid and the terminase packages the viral DNA into the procapsid until the next cos site on the concatemer reaches the complex. The downstream cos site is then cut generating the mature right end of the genome, the heterotrimer undocks from the DNA-filled head and remains bound to the left end of concatemer's next genome.
Subcellular Location
Host cytoplasm.
Protein Families
Terminase small subunit family
Database Links

KEGG: vg:2703523

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