EST2 Antibody

Code CSB-PA224389XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EST2 Polyclonal antibody
Uniprot No.
Target Names
EST2
Alternative Names
EST2 antibody; YLR318W antibody; L8543.12 antibody; Telomerase reverse transcriptase antibody; EC 2.7.7.49 antibody; Telomerase catalytic subunit antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EST2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.
Gene References into Functions
  1. Binding of the purified TEN domain (residues 1-161) of Est2 to telomeric DNA is enhanced by the E76K mutation. PMID: 22193961
  2. Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2. PMID: 21659533
  3. in the presence of manganese, both yeast and human telomerase can switch to a template- and RNA-independent mode of DNA synthesis, acting in effect as a terminal transferase. PMID: 15994230
  4. specific amino acids within region I of the catalytic subunit of telomerase play a previously unanticipated role in the response to Tel1p regulation at the telomere PMID: 16199886
  5. the finger subdomain of Est2p facilitates the action of Pif1p at telomeres. PMID: 16878131
  6. Est2p can directly or indirectly influence the binding of Rap1p to telomeric DNA, implicating telomerase in roles both upstream and downstream of Rap1p in telomere length homeostasis. PMID: 18212041

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Subcellular Location
Nucleus. Chromosome, telomere.
Protein Families
Reverse transcriptase family, Telomerase subfamily
Database Links

KEGG: sce:YLR318W

STRING: 4932.YLR318W

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