Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) tesA Polyclonal antibody

Uniprot No.

P0ADA1

Target Names

tesA

Alternative Names

tesA antibody; apeA antibody; pldC antibody; b0494 antibody; JW0483 antibody; Thioesterase 1/protease 1/lysophospholipase L1 antibody; TAP antibody; Acyl-CoA thioesterase 1 antibody; TESA antibody; EC 3.1.2.2 antibody; Acyl-CoA thioesterase I antibody; Arylesterase antibody; EC 3.1.1.2 antibody; Lysophospholipase L1 antibody; EC 3.1.1.5 antibody; Oleoyl-[acyl-carrier-protein] hydrolase antibody; EC 3.1.2.14 antibody; Phospholipid degradation C antibody; Pldc antibody; Protease 1 antibody; EC 3.4.21.- antibody; Protease I antibody; Thioesterase I/protease I antibody; TEP-I antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) tesA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

TesA is a multifunctional esterase that can act as a thioesterase, lysophospholipase and protease. TesA functions as a thioesterase specific for fatty acyl thioesters of greater than ten carbons, with highest activity on palmitoyl-CoA, cis-vaccenoyl-CoA and palmitoleoyl-CoA. TesA also possesses an arylesterase activity towards short acyl-chain aromatic esters such as alpha-naphthyl acetate, alpha-naphthyl butyrate, benzyl acetate and phenyl acetate. Also able to hydrolyze short acyl-chain triacylglycerols such as triacetin and tributyrin, and p-nitrophenyl esters such as p-nitrophenyl hexanoate and p-nitrophenyl butyrate. The protease activity is mainly active on small peptides. TesA is also able to hydrolyze p-nitrophenyl esters of N-substituted amino acids such as N-benzyloxycarbonyl-L-Phe-p-nitrophenyl ester (Z-L-Phe-ONp) and N-benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester (Z-L-Tyr-ONp), however it is unable to hydrolyze N-acetyl-L-Phe ethyl ester and its Tyr analog. TesA also hydrolyzes N-benzyloxycarbonyl-L-Phe beta-nitrophenyl ester (Cbz-Phe-ONap) and N-acetyl-DL-Phe-2-naphthyl ester (chymotrypsin-like specificity). Shows a slow proteolytic activity against denatured casein. The lysophospholipase activity of TesA is able to hydrolyze 1-palmitoyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoglycerol, 1- and 2-acyl-sn-glycero-3-phosphoethanolamine.

Gene References into Functions

Octanoic acid binding to the substrate-binding crevice of multifunctional tesA results in a continuous hydrophobic surface, which triggers switch loop movement essential for efficient catalysis of thioesters in tesA. PMID: 15697222
evaluated biochemical roles of amino acid residues in catalytic triad and oxyanion hole of Escherichia coli thioesterase I PMID: 16515533
conducted site-directed mutagenesis to change Trp into alanine (Ala), phenylalanine (Phe), or tyrosine (Tyr) to unveil the role of the Trp(23) indole ring PMID: 19540368

Subcellular Location

Periplasm.

Protein Families

'GDSL' lipolytic enzyme family

Database Links

KEGG: ecj:JW0483

STRING: 316385.ECDH10B_0451

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tesA Antibody

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