TGH Antibody

Code CSB-PA132900XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TGH Polyclonal antibody
Uniprot No.
Target Names
TGH
Alternative Names
TGH antibody; At5g23080 antibody; MYJ24.7 antibody; G patch domain-containing protein TGH antibody; Protein TOUGH antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TGH protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions as component of microRNA (miRNA) and small interfering RNA (siRNA) biogenesis. May assist DCL1 and DCL4 to efficiently process and/or recruit the precursors of miRNAs and siRNAs. In the miRNA biogenesis pathway, associates with the DCL1 complex that processes primary miRNAs (pri-miRNAs) into miRNAs. Binds pri-miRNAs and precursor miRNAs (pre-miRNAs). Is required for the interaction between pri-miRNAs and DRB1. Required for general proper plant growth and, in particular, initiation of vascular development. Interacts genetically with AMP1, a glutamate carboxypeptidase involved in the regulation of meristem function.
Gene References into Functions
  1. TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs. PMID: 22802657
  2. The evolutionarily conserved TOUGH (TGH) protein is a novel regulator required for Arabidopsis thaliana development. It plays a role in RNA binding or processing. [TOUGH] [TGH] PMID: 16024589
Subcellular Location
Nucleus speckle. Nucleus, nucleoplasm.
Tissue Specificity
Expressed in vasculature of cotyledons and leaves, young meristematic tissues, trichomes and pistils.
Database Links

KEGG: ath:AT5G23080

STRING: 3702.AT5G23080.1

UniGene: At.49050

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