TIC20-I Antibody

Code CSB-PA814106XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TIC20-I Polyclonal antibody
Uniprot No.
Target Names
TIC20-I
Alternative Names
TIC20-I; At1g04940; F13M7.5; F13M7.7; Protein TIC 20-I, chloroplastic; Translocon at the inner envelope membrane of chloroplasts 20-I; AtTIC20-I
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TIC20-I protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope. Seems to be specific for photosynthesis-related pre-proteins. Partially redundant with TIC20-IV, but not with TIC20-II or TIC20-V.
Gene References into Functions
  1. atTic20 functions similarly to other small alpha-helical integral membrane proteins, such as Tim23, that are involved in protein transport across membranes. PMID: 25551276
  2. Tic20 protein is much less abundant than Tic110 in envelope membranes. Tic20 forms high molecular weight complexes separately from Tic110. PMID: 21961525
  3. The inner envelope localized Tic20 proteins expose their C-termini to the chloroplast stroma. PMID: 21874592
  4. The tic20-I mutants exhibit albinism and severe defects in chloroplast biogenesis. PMID: 21395885
Subcellular Location
Plastid, chloroplast inner membrane; Multi-pass membrane protein.
Protein Families
Tic20 family
Tissue Specificity
Expressed in leaves, shoots and roots. High expression in mature photosynthetic tissues. Lower levels in non-photosynthetic tissues and roots.
Database Links

KEGG: ath:AT1G04940

STRING: 3702.AT1G04940.1

UniGene: At.46000

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