Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM10 Polyclonal antibody

Uniprot No.

P87108

Target Names

TIM10

Alternative Names

TIM10 antibody; MRS11 antibody; YHR005C-A antibody; YHR005BC antibody; Mitochondrial import inner membrane translocase subunit TIM10 antibody; Mitochondrial intermembrane protein MRS11 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM10 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. Compared to TIM9, it may function as a substrate sensor.

Gene References into Functions

Tim10 is protected from degradation by the protease Yme1 by Tim9. PMID: 26182355
formation of the hexamer is beneficial but not vital for TIM10 function, whilst the N-terminal substrate-binding region of Tim10 is essential in vivo PMID: 17618651
Assembly of the mitochondrial TIM9-TIM10 complex: a multi-step reaction with novel intermediates. PMID: 18022191
oxidative folding and mitochondrial import are kinetically competitive processes; efficiency of mitochondrial import of the small Tim proteins is controlled, at least partially in vitro, by the rate of oxidative folding PMID: 18076384
The structure of the yeast Tim9-Tim10 hexameric assembly determined to 2.5 A is reported and mutational analysis was performed to evaluate the specific roles of Tim9 and Tim10. PMID: 19037098
A region within the Tim9 and Tim10 precursors, consisting of only nine amino acid residues, functions as a signal for the engagement of substrate proteins with the Mia40 receptor. PMID: 19297525

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Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion intermembrane space.

Protein Families

Small Tim family

Database Links

KEGG: sce:YHR005C-A

STRING: 4932.YHR005C-A

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TIM10 Antibody

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Target Background