TIM50 Antibody

Code CSB-PA185723XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM50 Polyclonal antibody
Uniprot No.
Target Names
TIM50
Alternative Names
TIM50 antibody; YPL063W antibody; Mitochondrial import inner membrane translocase subunit TIM50 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TIM50 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.
Gene References into Functions
  1. crystal structure of Tim50(164-361) at 2.67 A resolution PMID: 26323300
  2. The C-terminal domain of Tim50 contributes to complex formation with Tim23. PMID: 25263020
  3. The presequence binding domain can bind to the core domain of Tim50 through the presequence binding region. PMID: 24462684
  4. the intermembrane space domain of Tim50 induced the Tim23 channel to close PMID: 16763150
  5. Tim50 and its association with TIM23 has a role in the transfer of protein precursors between the inner and outer mitochondrial membrane. PMID: 19144822

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Subcellular Location
Mitochondrion inner membrane; Single-pass membrane protein.
Protein Families
TIM50 family
Database Links

KEGG: sce:YPL063W

STRING: 4932.YPL063W

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