Timp Antibody

Code CSB-PA894223XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Timp Polyclonal antibody
Uniprot No.
Target Names
Timp
Alternative Names
Timp antibody; CG6281 antibody; Tissue inhibitor of metalloproteinase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Timp protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Metalloproteinase inhibitor that acts on both matrix metalloproteinases Mmp1 and Mmp2 in vitro. Complexes with metalloproteinases and irreversibly inactivates them by binding to their catalytic zinc cofactor. Required for wing maturation which is the final step in morphogenesis of the adult fly. Involved in the negative regulation of developmental tissue invasion for imaginal disk eversion during metamorphosis by inhibiting Mmp-mediated basement membrane (BM) degradation. Required for oogenesis and for the long-term maintainance of germarial structure and shape in the adult ovaries. Required for maintaining composition and biophysical properties of the extracellular matrix (ECM), and for the normal organization and cyst production of the germline stem cell (GSC) niche.
Gene References into Functions
  1. Our results demonstrating a crucial role for timp in tissue organization and gamete production thus provide a link between the regulation of ECM metabolism and tissue homeostasis. PMID: 26808525
  2. identification as endogenous inhibitor of the Drosophila matrix metalloproteinases PMID: 14567681
Subcellular Location
Secreted.
Protein Families
Protease inhibitor I35 (TIMP) family
Tissue Specificity
Expressed in heads of female and male adult flies. Expressed at the time of eclosion in unopened wings of adult flies. Strongly expressed at the tip of ovarian germarium region 1 where germline stem cells (GSCs) and cystoblasts reside and in region 2 of t
Database Links

KEGG: dme:Dmel_CG6281

STRING: 7227.FBpp0081722

UniGene: Dm.2453

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