tir Antibody

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Code	CSB-PA752031XA01EOD
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli O157:H7 tir Polyclonal antibody

Uniprot No.

Q7DB77

Target Names

tir

Alternative Names

tir antibody; espE antibody; Z5112 antibody; ECs4561 antibody; Translocated intimin receptor Tir antibody; Secreted effector protein Tir antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli O157:H7

Immunogen

Recombinant Escherichia coli O157:H7 tir protein

Immunogen Species

Escherichia coli O157:H7

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain EDL933, acts via the effector protein EspF(U), in a phosphotyrosine- and NCK-independent manner. Tir binds to host BAIAP2, which mediates association with EspF(U) and leads to stimulation of actin polymerization.

Gene References into Functions

identified a 6 amino acid (519-524) carboxy-terminal region which was required for efficient Tir secretion and translocation; data suggest that this region may play a role in maintaining EHEC O157:H7 Tir in a secretion-competent conformation PMID: 15686835
localizes at or near the site of enterohemorrhagic Escherichia coli O157:H7 adherence to enterocytes in piglets, neonatal calves, and mice. PMID: 16428775

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein. Note=Secreted via the type III secretion system (TTSS). Released into the host cytoplasm via TTSS and then independently inserts into the plasma membrane from a cytoplasmic location. In host cells, localizes to the tip of the actin pedestal.

Protein Families

Tir receptor family

Database Links

KEGG: ece:Z5112

STRING: 155864.Z5112

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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tir Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background