tir Antibody

Datasheet

Code	CSB-PA485638XA01EOB
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli O127:H6 (strain E2348/69 / EPEC) tir Polyclonal antibody

Uniprot No.

B7UM99

Target Names

tir

Alternative Names

tir antibody; espE antibody; E2348C_3941 antibody; Translocated intimin receptor Tir antibody; Secreted effector protein Tir antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli O127:H6 (strain E2348/69 / EPEC)

Immunogen

Recombinant Escherichia coli O127:H6 (strain E2348/69 / EPEC) tir protein

Immunogen Species

Escherichia coli O127:H6 (strain E2348/69 / EPEC)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion. In strain E2348/69, acts mainly via the host adaptor proteins NCK1 and NCK2. Once clustered and phosphorylated at Tyr-474, Tir binds to NCK proteins, which in turn bind and activate host WASL/N-WASP, leading to actin polymerization. Can also trigger an inefficient, NCK-independent pedestal formation. This pathway involves phosphorylation of Tyr-454 and probably a putative host adaptor. Acts also via direct binding to the host cytoskeletal protein alpha-actinin in a NCK- and phosphotyrosine-independent manner. This interaction may stabilize the pedestal, but is not essential for its formation.

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein. Note=Secreted via the type III secretion system (TTSS). Released into the host cytoplasm via TTSS and then independently inserts into the plasma membrane from a cytoplasmic location. In host cells, localizes to the tip of the actin pedestal.

Protein Families

Tir receptor family

Database Links

KEGG: ecg:E2348C_3941

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TWIK2 channel mediates inflammation, study finds Osteoprotegerin (OPG): What is Its Potential as a Biomarker or Therapeutic Target? Neuronal Markers and Neurodegenerative Diseases Lysosomal Enzymes and Lysosomal Storage Disorders (LSDs) NKG2D, a Powerful Molecule Involved In CAR-T And Car-NK Cell Therapy Recombinant Monkeypox Virus Proteins - Accelerate Monkeypox Research A Comprehensive Overview of Ovarian Cancer Effects of FSHR on Fertility and Tumors The Overview of Recombinant Antibody The Overview of Cell Viability One World, One Hope——What Can We Do about AIDS? LPS/TLR4 Signal Transduction Pathway Toll-like Receptors--The Eye of Innate Immunity A Comprehensive Exploration into Amyotrophic Lateral Sclerosis CD47, A New Target for Tumor Immune GIPR: the Crucial Regulator of Glucose and Lipid Metabolism in Diabetes and Obesity! How to Measure Histone Modification? What A Paradoxical TNF Signaling Pathway-Based on Different Receptors SIGIRR: A Novel Member of the TIR Superfamily Acts as the Anti-Inflammatory 'Brake' to Control Immune Response in Various Diseases! TMPRSS2, an Activator for SARS-CoV-2 Invasion The Overview of Chemokine Serotransferrin/TF: a Major Transferrin in Plasma, a Novel TF-Modified Nanoparticle for Tumor-Targeting Research! Do You Really Know Neurons and Glia Cells in Nerve System? Cadherin-17 (CDH17): A Viable Target in Bispecific Antibodies or CAR-T Therapy to Fight Many Gastrointestinal Cancers! The Overview of Interferon The Overview of Organelles The Overview of Breast Cancer: Related Signaling Pathways, Therapeutic Targets The Encyclopedia of Common Virus The Review of Post-translational Modifications CXCR4: An Attractive Target of GPCR family Brings Promising New Drugs for Cancer Therapy！ IL-6: an Important Participant in the Inflammatory Cascade, One of the Most Functional Cytokines in Various Diseases Research! Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

tir Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background