unc-94 Antibody

Code CSB-PA515310XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans unc-94 Polyclonal antibody
Uniprot No.
Target Names
unc-94
Alternative Names
unc-94 antibody; tmd-1 antibody; C06A5.7 antibody; Tropomodulin antibody; Tmod-like protein antibody; cTmd1 antibody; Uncoordinated protein 94 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans unc-94 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as the pointed end capping protein which maintains the length and dynamics of the actin filament. Blocks the elongation and depolymerization of the actin filaments at the pointed end.
Gene References into Functions
  1. The data support a model in which minus-end actin capping by UNC-94 promotes proper F-actin structure and contraction in the terminal web, yielding proper shape of the intestinal lumen. PMID: 24677443
  2. In vitro, UNC-94 acts in combination with HMP-1, leading to longer actin bundles than with HMP-1 alone. PMID: 22771044
  3. C-terminal part of tropomodulin protein 1, isoform A, from Caenorhabditis elegans was expressed in Escherichia coli and purified to homogeneity PMID: 12777789
  4. cTmd1 consists of AAs 196-392. The chain is well defined in the 2Fo-Fc map for the range 222-388. Much of the N-terminus, including AAs 196-221, is entirely disordered. The C-terminus of cTmd1 has 6 additional AAs. 4 of them (389-392) are disordered. PMID: 15211521
  5. study shows that the mutationally identified gene unc-94 corresponds with the sequence-predicted gene tmd-1 (C06A5.7), which encodes a tropomodulin PMID: 17976644

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Tropomodulin family
Database Links

KEGG: cel:CELE_C06A5.7

STRING: 6239.C06A5.7b

UniGene: Cel.17356

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