Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Tollo Polyclonal antibody

Uniprot No.

Q9V477

Target Names

Tollo

Alternative Names

Tollo antibody; Tl-8 antibody; Toll-8 antibody; CG6890 antibody; Toll-like receptor Tollo antibody; EC 3.2.2.6 antibody; Toll-like receptor 8 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Tollo protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Toll-related receptor. Probably specific to larval innate immunity. Involved in the tracheal immune response of larvae to Gram-negative and perhaps Gram-positive bacteria; upon infection it negatively regulates the immune deficiency (Imd) signaling cascade specifically in the respiratory epithelium to prevent the overexpression of antimicrobial peptides (AMP). Involved in the NF-kappa-B-dependent apoptosis of unfit cells during cell competition. Involved in neuron-specific glycosylation. Positively controls the neuromuscular junction (NMJ) growth in presynaptic motorneurons, probably via the JNK pathway. During development of the peripheral nervous system, may function in the NF-kappa-B (rel) regulatory cascade to repress expression of the neuron-specific genes sc and ase in non-neuronal cells. Promotes heterophilic cell adhesion with 18w in vitro. May have a minor role in leg development. May be involved in determining the proximal cell fate in the wing, possibly by negatively regulating the Dpp signaling pathway.(PubMed:17078066). May also be involved in the Dpp signaling pathway in the eye. Possibly functions with 18w and Toll-6 during convergent extension, to help direct proper planar cell polarity, cell intercalation and axis elongation.

Gene References into Functions

Tollo expression in the motoneuron is both necessary and sufficient for regulating neuromuscular junction growth. PMID: 24662564
findings show Toll-8 (Tollo) plays an important role in controlling the respiratory epithelium immune response; data indicate that, by antagonizing the IMD pathway, Tollo is preventing over-activation of the antibacterial response in the airway epithelium PMID: 22022271
Ectopic expression of Tollo/Toll-8 antagonizes Dpp signaling and induces cell sorting in the Drosophila wing. PMID: 17078066
tollo/toll-8 signaling influences flux through several processing steps that affect the maturation of N-linked glycans PMID: 17264077

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Apical cell membrane.

Protein Families

Toll-like receptor family

Database Links

KEGG: dme:Dmel_CG6890

STRING: 7227.FBpp0075360

UniGene: Dm.3403

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Tollo Antibody

Product Details

Customer Reviews and Q&A

Target Background