Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TOM22 Polyclonal antibody

Uniprot No.

P49334

Target Names

TOM22

Alternative Names

TOM22 antibody; MAS17 antibody; MAS22 antibody; MOM22 antibody; YNL131W antibody; N1217 antibody; N1862 antibody; Mitochondrial import receptor subunit TOM22 antibody; Mitochondrial 17 kDa assembly protein antibody; Mitochondrial 22 kDa outer membrane protein antibody; Protein MAS17 antibody; Translocase of outer membrane 22 kDa subunit antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TOM22 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.

Gene References into Functions

Tom22 is regulated by the cytosolic kinases CK2 and PKA and by membranebound CK1. PMID: 24093680
Study shows that the cytosolic domain of Tom22 links the outer mitochondrial membrane and the sorting and assembly machinery into a supercomplex, facilitating precursor transfer on the intermembrane space side and reveals receptor-mediated coupling of import and export translocases as a means of precursor channeling. PMID: 23911324
We also identified a core sequence of Tom22 that binds to Tim21. Furthermore, negatively charged amino-acid residues of Tom22 are important for binding to Tim21. Here we suggest a mechanism for the TOM-TIM interaction. PMID: 17099692
Data Show that Tom22 are anchored in the outer membrane by a single transmembrane alpha-helix, located in the C-terminal portion of Tom22 (tail-anchored) and insertion of the precursor of Tom22 into the outer membrane requires pre-existing Tom receptors. PMID: 17974559
beta2GP1-dependent uptake is mediated by bridging of the target cell to the phagocyte through the protein C- and N-terminal domains, respectively PMID: 18063580

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Subcellular Location

Mitochondrion outer membrane; Single-pass type II membrane protein.

Protein Families

Tom22 family

Database Links

KEGG: sce:YNL131W

STRING: 4932.YNL131W

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TOM22 Antibody

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Target Background