topA Antibody

Code CSB-PA024056XA01BRB
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Product Details

Full Product Name
Rabbit anti-Bacillus pseudofirmus (strain OF4) topA Polyclonal antibody
Uniprot No.
Target Names
topA
Alternative Names
topA antibody; BpOF4_21279/BpOF4_21284 antibody; DNA topoisomerase 1 antibody; EC 5.6.2.1 antibody; DNA topoisomerase I antibody; Omega-protein antibody; Relaxing enzyme antibody; Swivelase antibody; Untwisting enzyme antibody
Raised in
Rabbit
Species Reactivity
Bacillus pseudofirmus (strain OF4)
Immunogen
Recombinant Bacillus pseudofirmus (strain OF4) topA protein
Immunogen Species
Bacillus pseudofirmus (strain OF4)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
Protein Families
Type IA topoisomerase family
Database Links
CUSABIO guaranteed quality
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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