top-2 Antibody

Code CSB-PA630491XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans top-2 Polyclonal antibody
Uniprot No.
Target Names
top-2
Alternative Names
top-2 antibody; K12D12.1 antibody; DNA topoisomerase 2 top-2 antibody; EC 5.6.2.2 antibody; DNA topoisomerase II antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans top-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes. Required for centromere resolution during mitosis. Required for chromosome segregation in anaphase of meiosis I during spermatogenesis. Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions. Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response. Essential for embryogenesis.
Gene References into Functions
  1. Topo-II promotes chromosome-length shortening. PMID: 28733327
  2. These results explain why hTOPIIalpha and hTOPIIbeta are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses. PMID: 28700616
  3. Novel Top-2 mutation disrupts chromosome segregation during spermatogenesis. PMID: 27707787
Subcellular Location
Nucleus, nucleoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle.
Protein Families
Type II topoisomerase family
Tissue Specificity
Expressed in the hermaphrodite and male germline.
Database Links

KEGG: cel:CELE_K12D12.1

STRING: 6239.K12D12.1

UniGene: Cel.6930

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