TOP3 Antibody

Datasheet
Code CSB-PA527651XA01SXV
Product Type Polyclonal Antibody
Uniprot No. O60126
Delivery Make-to-order
Protocols ELISA Protocol
Immunogen Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) TOP3 protein
Raised in Rabbit
Species Reactivity Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Tested Applications ELISA, WB (ensure identification of antigen)
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Protein A/G
Isotype IgG
Clonality Polyclonal
Alias DNA topoisomerase 3 (EC 5.99.1.2) (DNA topoisomerase III) top3 SPBC16G5.12c
Immunogen Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Gene Names top3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Protein Families Type IA topoisomerase family
Database Links

KEGG: spo:SPBC16G5.12c

STRING: 4896.SPBC16G5.12c.1

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