Top3alpha Antibody

Datasheet

Code	CSB-PA865075XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Top3alpha Polyclonal antibody

Uniprot No.

Q9NG98

Target Names

Top3alpha

Alternative Names

Top3alpha antibody; CG10123 antibody; DNA topoisomerase 3-alpha antibody; EC 5.6.2.1 antibody; DNA topoisomerase III alpha antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Top3alpha protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.

Gene References into Functions

The results reported here demonstrate that mitochondrial Top3alpha ablation results in mitochondrial genome instability and its dysfunction, thereby accelerating the aging process. PMID: 27067525
the Top3alpha C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA) and an essential function in vivo. PMID: 22511792
described the phenotypes associated with mitochondrial dysfunction, including the female sterility and the loss of germ-line stem cells of males, as a result from topo IIIalpha deprivation. PMID: 20308575
partially relaxes a hypernegatively supercoiled plasmid substrate consistent with other purified topo IIIs. PMID: 15537633
Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration PMID: 16849422

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Protein Families

Type IA topoisomerase family

Database Links

KEGG: dme:Dmel_CG10123

STRING: 7227.FBpp0080787

UniGene: Dm.2659

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Top3alpha Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background