Top3beta Antibody

Code CSB-PA530977XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Top3beta Polyclonal antibody
Uniprot No.
Target Names
Top3beta
Alternative Names
Top3beta antibody; TOP3 antibody; CG3458 antibody; DNA topoisomerase 3-beta antibody; EC 5.6.2.1 antibody; DNA topoisomerase III beta antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Top3beta protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.
Gene References into Functions
  1. These results demonstrate that TDRD3 can enhance the biochemical activities of Top3beta on both DNA and RNA substrates, in addition to its function of targeting Top3beta to critical sites in subcellular compartments. PMID: 27582462
  2. The results of this study suggested that Top3beta acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for synapse formation. PMID: 23912945
  3. studies suggest that the topo III enzyme behaves as a structure-specific endonuclease in vivo, providing a reversible DNA cleavage activity that is specific for unpaired regions in the DNA PMID: 12048241
Protein Families
Type IA topoisomerase family
Database Links

KEGG: dme:Dmel_CG3458

STRING: 7227.FBpp0070891

UniGene: Dm.2946

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