Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Topors Polyclonal antibody

Uniprot No.

Q9V8P9

Target Names

Topors

Alternative Names

Topors antibody; CG15104 antibody; E3 ubiquitin-protein ligase Topors antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase Topors antibody; SUMO1-protein E3 ligase Topors antibody; Topoisomerase I-binding RING finger protein antibody; Topoisomerase I-binding arginine/serine-rich protein antibody; dTopors antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Topors protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Functions as a ubiquitin-protein E3 ligase. Negatively regulates the transcriptional repressor h/hairy by promoting its ubiquitination and subsequent degradation. Also directs the nuclear organization of the gypsy chromatin insulator. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them, and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may require the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may promote formation of nuclear insulator bodies by recruiting individual insulator complexes to the nuclear lamina.

Gene References into Functions

dtopors plays a structural role in spermatocyte lamina that is critical for multiple aspects of meiotic chromosome transmission PMID: 21900273
Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein PMID: 14871887

Subcellular Location

Nucleus. Chromosome. Note=Colocalizes with the gypsy chromatin insulator complex on polytene chromosomes and to nuclear insulator bodies. Also localizes to the nuclear lamina.

Database Links

KEGG: dme:Dmel_CG15104

STRING: 7227.FBpp0304302

UniGene: Dm.10778

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Topors Antibody

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