Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tor2 Polyclonal antibody

Uniprot No.

Q9Y7K2

Target Names

tor2

Alternative Names

tor2 antibody; SPBC216.07c antibody; SPBC646.01c antibody; Serine/threonine-protein kinase tor2 antibody; EC 2.7.11.1 antibody; Phosphatidylinositol kinase homolog tor2 antibody; Target of rapamycin kinase 2 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tor2 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Phosphatidylinositol 3-kinase homolog, component of TORC1, which regulates multiple cellular processes to control cell growth in response to environmental signals. TORC1 controls the switch between cell proliferation and differentiation by sensing nutrient availability. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls cell growth and ribosome biogenesis by regulating ribosomal protein gene expression. In nutrient rich conditions, responsible for the phosphorylation of AGC S6 kinase (S6K) psk1 at 'Thr-392' and 'Thr-415', activating psk1 kinase activity and promoting phosphorylation of ribosomal protein S6. Also catalyzes the nutrient-dependent phosphorylation of sck1 and sck2. TORC1 negatively controls G1 cell-cycle arrest, sexual development and amino acid uptake. Represses mating, meiosis and sporulation efficiency by interfering with the functions of the transcription factor ste11 and the meiosis-promoting RNA-binding protein mei2.

Gene References into Functions

These results suggest that nitrogen depletion decreases Tor2 activity through Tsc2, thereby promoting the surface expression of Agp3 from trans-Golgi/endosomes. PMID: 26447710
Rapamycin inactivates Tor2 activity in the presence of caffeine. PMID: 22762302
Rapamycin inhibits the ability of TORC1 to phosphorylate Rps6, owing to interaction of the rapamycin-FKBP12 complex with the FRB domain in Tor2 PMID: 20144990
Studies postulate that the signaling cascade may branch below Rhb1 or Tor2 and regulate the amino acid uptake and response to nitrogen starvation independently. PMID: 19620394
Target of rapamycin complex 2 (TORC2) is not essential for cell viability in S. pombe but plays important roles in cellular survival of stress conditions through phosphorylation and activation of an AGC-family protein kinase, Gad8. PMID: 18235227

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Subcellular Location

Cytoplasm.

Protein Families

PI3/PI4-kinase family

Database Links

KEGG: spo:SPBC216.07c

STRING: 4896.SPBC216.07c.1

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tor2 Antibody

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