TPL Antibody

Code CSB-PA856722XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TPL Polyclonal antibody
Uniprot No.
Target Names
TPL
Alternative Names
TPL antibody; WSIP1 antibody; At1g15750 antibody; F7H2.9 antibody; Protein TOPLESS antibody; WUS-interacting protein 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TPL protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional corepressor. May repress the expression of root-promoting genes in the top half of the embryo to allow proper differentiation of the shoot pole during the transition stage of embryogenesis. Regulates the expression of PLT1 and PLT2. Negative regulator of jasmonate responses. Negative regulator of auxin responses. Negative regulator of multiple floral organ identity genes. Required for ovule development.
Gene References into Functions
  1. relative to mammalian TBL1, evolved a new tetramerization interface and unique and highly conserved surface for interaction with repressors PMID: 28698367
  2. The GIR1 and GIR2 might may link TPL to and participate in epigenetic regulation of root hair development. PMID: 28526412
  3. These data unveil a pivotal role of the co-repressor TPL in the shoot and root meristems. PMID: 28320734
  4. TOPLESS mediates brassinosteroid-induced transcriptional repression through interaction with BZR1. PMID: 24938363
  5. Topless and Topless-related proteins form a tertiary complex with TIE1 protein at early stages of leaf development. PMID: 23444332
  6. We identified transcription factors interacting with TPL. PMID: 22065421
  7. Arabidopsis JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA) PMID: 20360743
  8. TPL and other TPR proteins are necessary to repress the expression of root-promoting genes in the top half of the embryo to allow proper differentiation of the shoot pole. [topless][TPL] PMID: 16763149
  9. TPL can interact with IAA12/BODENLOS through an ETHYLENE RESPONSE FACTOR-associated amphiphilic repression motif; study shows TPL is a transcriptional co-repressor & furthers understanding of how auxin regulates transcription during plant development PMID: 18258861

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in embryo and in extraembryonic tissues. Expressed in inflorescences, flowers, floral meristems, developing anthers and ovules. Detected in the vascular tissues, shoot apical meristem, cotyledons and young leaves. Expressed ubiquitously in the p
Database Links

KEGG: ath:AT1G15750

STRING: 3702.AT1G15750.1

UniGene: At.48194

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