TPO1 Antibody

Code CSB-PA947022XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TPO1 Polyclonal antibody
Uniprot No.
Target Names
TPO1
Alternative Names
TPO1 antibody; YLL028W antibody; L0939 antibody; Polyamine transporter 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TPO1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Catalyzes polyamine uptake at alkaline pH and excretion at acidic pH. Recognizes spermidine, spermine and putrescine, the polyamine analogs methylglyoxal bis(guanylhydrazone) (MGBG) and paraquat, the antimalarial drug quinidine, and cycloheximide. Confers resistance to the non-steroidal anti-inflammatory drug indomethacin.
Gene References into Functions
  1. results described suggest that Tpo1 is one of the key players standing in the crossroad between benzoic acid stress response and tolerance and the control of the intracellular concentration of nitrogenous compounds PMID: 28409382
  2. The present study provides evidence that alterations in the sphingolipid level/structure result in loss of Tpo1 protein from the mother at each cell division leading to a progressive decline in the total protein level in mother. PMID: 28768828
  3. TPO1 times induction of oxidation stress response proteins and cell cycle arrest. PMID: 24136413
  4. YAP1 overexpression rescues the imp2Delta mutant from bleomycin toxicity by triggering Tpo1 expression to expel the drug. PMID: 24599794
  5. The timing of TPO1 expression during the cell cycle is critical to the observed localization patterns, asymmetric localization of Tpo1 to the mother cortex. PMID: 20657593
  6. The transport activity of TPO1 was enhanced through phosphorylation at Ser19 by protein kinase C and at Thr52 by casein kinase 1 PMID: 15637075
  7. The transcriptional activation of TPO1 and PDR5 was registered after exposure of the unadapted yeast population to acute artesunate-induced stress, being significantly reduced in the absence of Pdr1p and abolished in the absence of Pdr1p and Pdr3p. PMID: 17156010
  8. We screened for Saccharomyces cerevisiae genes whose overexpression causes indomethacin resistance and identified the TPO1 gene, which encodes a major facilitator superfamily transporter. PMID: 17362938

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Enriched on the cell membrane of growing buds.
Protein Families
Major facilitator superfamily, DHA1 family, Polyamines/proton antiporter (TC 2.A.1.2.16) subfamily
Database Links

KEGG: sce:YLL028W

STRING: 4932.YLL028W

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