Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tpx1 Polyclonal antibody

Uniprot No.

O74887

Target Names

tpx1

Alternative Names

tpx1 antibody; tsa1 antibody; SPCC576.03c antibody; Peroxiredoxin tpx1 antibody; EC 1.11.1.15 antibody; Thioredoxin peroxidase antibody; TPx antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) tpx1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Relays hydrogen peroxide as a signal to the transcription factor pap1 by inducing the formation of intramolecular disulfide bonds in pap1, which causes its nuclear accumulation and activation. Reduced by srx1 and this regulation acts as a molecular switch controlling the transcriptional response to hydrogen peroxide.

Gene References into Functions

our data establish that redox-coupling between Trx1 and the thioredoxin peroxidase activity of Tpx1 is central for initiation of appropriate responses to different levels of hydrogen peroxide in S. pombe PMID: 22245228
Thioredoxin Peroxidase inhibited thermal aggregation of citrate synthase, but BCP failed to inhibit the aggregation. PMID: 20356456
redox regulation of the thioredoxin peroxidase activity of Tpx1 acts as a molecular switch controlling the transcriptional response to H(2)O(2) PMID: 15824112
The peroxiredoxin Tpx1 is an upstream activator of Pap1. Tpx1 oxidation to the cysteine-sulfinic acid constitutes a previously uncharacterized redox switch in H2O2 signaling, restricting Pap1 activation within a narrow range of H2O2 concentrations. [Tpx1] PMID: 15956211
Prx protein is partly responsible for maintaining low ROS level under normal and stressful growth conditions in the fission yeast PMID: 17530441
the carboxy-terminal extension of Tpx1 concomitantly improves H2O2 scavenging and increases susceptibility to inactivation PMID: 18233959

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peroxiredoxin family, AhpC/Prx1 subfamily

Database Links

KEGG: spo:SPCC576.03c

STRING: 4896.SPCC576.03c.1

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tpx1 Antibody

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