TRM112 Antibody

Code CSB-PA347430XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TRM112 Polyclonal antibody
Uniprot No.
Target Names
TRM112
Alternative Names
TRM112 antibody; YNR046W antibody; N3445 antibody; Multifunctional methyltransferase subunit TRM112 antibody; eRF1 methyltransferase subunit TRM112 antibody; eRF1 MTase subunit TRM112 antibody; tRNA methyltransferase 112 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TRM112 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase MTQ2, required for the methylation of eRF1 on 'Gln-182'. Together with methyltransferase TRM11, required for the formation of 2-methylguanosine at position 10 (m2G10) in tRNA. Together with methyltransferase BUD23, required for the formation of 7-methylguanine at position 1575 (m7G1575) in 18S rRNA. Involved in biogenesis of both 40S and 60S ribosomal subunits.
Gene References into Functions
  1. Trm112 is required for efficient ribosome synthesis and progression through mitosis PMID: 22493060
  2. Trm112p is a 15-kDa zinc finger protein essential for the activity of two tRNA and one protein methyltransferases in yeast PMID: 20400505
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TRM112 family
Database Links

KEGG: sce:YNR046W

STRING: 4932.YNR046W

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