trfA Antibody

Code CSB-PA357176XA01ENL
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Product Details

Full Product Name
Rabbit anti-Escherichia coli trfA Polyclonal antibody
Uniprot No.
Target Names
trfA
Alternative Names
trfA antibody; Plasmid replication initiator protein TrfA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli trfA protein
Immunogen Species
Escherichia coli
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for initiation of plasmid DNA replication, along with host-derived DnaA and other host proteins. Both forms of the protein are capable of initiating plasmid replication in a number of Gram-negative bacteria. Binds to 8 17-base pair repeat sequences (iterons) in the RK2 minimal replication origin (oriV), opening the origin of replication. oriV opening does not absolutely require the presence of nucleotides; formation of open complex is somewhat enhanced by ATP or ATP gamma S, while DnaA or HU is required for full opening.; Also involved in plasmid copy number control, promoting intermolecular coupling of protein bound iterons at oriV, which inhibits replication initiation.
Subcellular Location
Cell inner membrane; Peripheral membrane protein. Note=Probably the site where plasmid replication is initiated.
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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