GCD14 Antibody

Code CSB-PA344421XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCD14 Polyclonal antibody
Uniprot No.
Target Names
GCD14
Alternative Names
GCD14 antibody; TRM61 antibody; YJL125C antibody; J0710 antibody; tRNA antibody; adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 antibody; EC 2.1.1.220 antibody; General control non-derepressible protein 14 antibody; Protein GCD14 antibody; tRNA(m1A58)-methyltransferase subunit TRM61 antibody; tRNA(m1A58)MTase subunit TRM61 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GCD14 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.
Gene References into Functions
  1. Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 has been reported. PMID: 27582183
  2. X-ray diffraction analysis of the TRM6-TRM61 complex were performed in this study. The crystals diffracted to 2.80 A resolution and belonged to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 139.14, c = 101.62 A PMID: 22102254
Subcellular Location
Nucleus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, TRM61 family
Database Links

KEGG: sce:YJL125C

STRING: 4932.YJL125C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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