trmL Antibody

Code CSB-PA936711XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) trmL Polyclonal antibody
Uniprot No.
Target Names
trmL
Alternative Names
trmL antibody; yibK antibody; b3606 antibody; JW3581 antibody; tRNA antibody; cytidine(34)-2'-O)-methyltransferase antibody; EC 2.1.1.207 antibody; tRNA antibody; cytidine/uridine-2'-O-)-methyltransferase TrmL antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) trmL protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37.
Gene References into Functions
  1. Data show that only pyrimidines at the wobble position are recognized by C/U34 2'-O-methyltransferase (EcTrmL). PMID: 26106808
  2. The results suggest that TrmL functions as a homodimer by using the conserved C-terminal half of the SPOUT domain for catalysis, whereas residues from the less-conserved N-terminal half of the other subunit participate in tRNA recognition. PMID: 23804755
  3. Loss of YibK methylation reduces the efficiency of codon-wobble base interaction, as demonstrated in an amber suppressor supP system. PMID: 20855540
Subcellular Location
Cytoplasm.
Protein Families
Class IV-like SAM-binding methyltransferase superfamily, RNA methyltransferase TrmH family, TrmL subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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