ASA1 Antibody

Code CSB-PA335695XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ASA1 Polyclonal antibody
Uniprot No.
Target Names
ASA1
Alternative Names
ASA1 antibody; AMT1 antibody; JDL1 antibody; TRP5 antibody; WEI2 antibody; At5g05730 antibody; MJJ3.14 antibody; Anthranilate synthase alpha subunit 1 antibody; chloroplastic antibody; EC 4.1.3.27 antibody; Anthranilate synthase component 1-1 antibody; Anthranilate synthase component I-1 antibody; Protein A-METHYL TRYPTOPHAN RESISTANT 1 antibody; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1 antibody; Protein TRYPTOPHAN BIOSYNTHESIS 5 antibody; Protein WEAK ETHYLENE INSENSITIVE 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ASA1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS to produce anthranilate. Plays an important regulatory role in auxin production via the tryptophan-dependent biosynthetic pathway.
Gene References into Functions
  1. indole-3-butyric acid induced adventitious root-formation by conversion into Indole-3-acetic acid (IAA) involving nitric oxide activity, and by a positive action on IAA-transport and ASA1/ASB1-mediated IAA-biosynthesis PMID: 28693423
  2. ASA1 has a role in jasmonate-induced auxin biosynthesis. PMID: 19435934
  3. Pore mutations in ammonium transporter AMT1 may have a role in increased electrogenic ammonium transport activity PMID: 19581303
Subcellular Location
Plastid, chloroplast.
Protein Families
Anthranilate synthase component I family
Tissue Specificity
Expressed in the central cylinder of mature primary root zones, including pericycle and early lateral root primordia, and vasculature of cotyledons.
Database Links

KEGG: ath:AT5G05730

STRING: 3702.AT5G05730.2

UniGene: At.285

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