trpE Antibody

Code CSB-PA360591XA01SXB
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Product Details

Full Product Name
Rabbit anti-Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) trpE Polyclonal antibody
Uniprot No.
Target Names
trpE
Alternative Names
trpE antibody; STM1723 antibody; Anthranilate synthase component 1 antibody; AS antibody; ASI antibody; EC 4.1.3.27 antibody
Raised in
Rabbit
Species Reactivity
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Immunogen
Recombinant Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) trpE protein
Immunogen Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.
Gene References into Functions
  1. The tryptophan biosynthetic enzyme complex anthranilate synthase-phosphoribosyltransferase, composed of the TrpD and TrpE proteins, was shown to be essential for phosphoribosyl amine formation in strains lacking both yjgF and purF. PMID: 16980480
Protein Families
Anthranilate synthase component I family
Database Links

KEGG: stm:STM1723

STRING: 99287.STM1723

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