NTRC Antibody

Code CSB-PA521234XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NTRC Polyclonal antibody
Uniprot No.
Target Names
NTRC
Alternative Names
NTRC antibody; At2g41680 antibody; T32G6.20 antibody; NADPH-dependent thioredoxin reductase 3 antibody; NTR3 antibody; EC 1.8.1.9 antibody; NADPH-dependent thioredoxin reductase C antibody; ANTR-C antibody; AtNTRC antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NTRC protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Thioredoxin reductase (TR) that exhibits both TR and thioredoxin (Trx) activities. Contains a C-terminal functional Trx domain. Functions as an electron donor for plastidial 2-Cys peroxiredoxins and participates in a NADPH-dependent hydrogen peroxide scavenging system in chloroplasts in the dark. Required for chlorophyll biosynthesis and biogenesis of the photosynthetic apparatus. Activates aerobic cyclase which converts Mg-protoporhyrin monomethyl ester into protochlorophyllide. Involved in a light-dependent regulation of starch biosynthesis by redox activation of the ADP-glucose pyrophosphorylase (AGPase), a central enzyme of starch synthesis.
Gene References into Functions
  1. NTRC plays a pivotal role in chloroplast redox regulation, being necessary for the activity of diverse thioredoxins with unrelated functions. PMID: 28500266
  2. NTRC-mediated regulation of the Calvin-Benson cycle and ATP synthesis occurs both directly and through interaction with the ferredoxin-thioredoxin system and is crucial when availability of light is limiting photosynthesis. PMID: 26831830
  3. NTRC is highly sensitive to rapidly changing light intensities.NTRC is not responsible for 'metabolism-related' regulation of the ATP synthase. PMID: 27233821
  4. cooperative control of chloroplast functions via the FTR/Trx and NTRC pathways is essential for plant viability PMID: 27335455
  5. These data uncover a new role for NTRC in the control of photosynthetic yield. PMID: 26476233
  6. An Arabidopsis thaliana double knockout mutant lacking NTRC and Srx shows a phenotype similar to the ntrc mutant, while the srx mutant resembles wild-type plants. NTRC deficiency causes reduced overoxidation of 2-Cys peroxiredoxins. PMID: 25560178
  7. NADPH thioredoxin reductase C is involved in redox regulation of the Mg-chelatase I subunit in Arabidopsis thaliana chloroplasts PMID: 24658415
  8. interaction of chloroplast 2-Cys peroxiredoxin with NADPH-thioredoxin reductase C (NTRC) and thioredoxin x PMID: 25448674
  9. The strongest reduction in ntrc growth occurred under photoperiods with nights longer than 14 h, whereas knockout of the NTRC gene did not alter the circadian-clock-controlled growth. Lack of NTRC modulated chloroplast reactive oxygen species metabolism. PMID: 23881397
  10. heat shock-mediated holdase chaperone function of NTRC is responsible for the increased thermotolerance of Arabidopsis and the activity is significantly supported by NADPH PMID: 23024205
  11. analysis of electron transfer pathways and dynamics of chloroplast NADPH-dependent thioredoxin reductase C (NTRC) PMID: 22833674
  12. NADPH-thioredoxin reductase C (NTRC), previously reported as exclusive to green tissues, is also expressed in nonphotosynthetic tissues of Arabidopsis thaliana. PMID: 22505729
  13. ANTR-C functions as an electron donor for plastidial 2-Cys peroxiredoxins and represents the NADPH-dependent thioredoxin/thioredoxin reductase system in chloroplasts. [ANTR-C] PMID: 16884685
  14. Arabidopsis knock-out mutant showed growth inhibition and hypersensitivity to methyl viologen. PMID: 15292215
  15. NTRC and 2-Cys Prx are involved in the protection of at least the later part of the chlorophyll biosynthetic pathway; NTRC and 2-Cys Prx in vitro stimulate the aerobic cyclase step of this pathway. PMID: 18625226
  16. Chloroplast NADPH-thioredoxin reductase, NTRC, regulates several key processes, including chlorophyll biosynthesis and the shikimate pathway, in chloroplasts. [NTRC] PMID: 19151130

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Subcellular Location
Plastid, chloroplast.
Protein Families
Class-II pyridine nucleotide-disulfide oxidoreductase family
Database Links

KEGG: ath:AT2G41680

STRING: 3702.AT2G41680.1

UniGene: At.12409

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