tsaD Antibody

Code CSB-PA356643XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) tsaD Polyclonal antibody
Uniprot No.
Target Names
tsaD
Alternative Names
tsaD antibody; gcp antibody; ygjD antibody; b3064 antibody; JW3036 antibody; tRNA N6-adenosine threonylcarbamoyltransferase antibody; EC 2.3.1.234 antibody; N6-L-threonylcarbamoyladenine synthase antibody; t(6)A synthase antibody; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD antibody; tRNA threonylcarbamoyladenosine biosynthesis protein TsaD antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) tsaD protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. May also be involved in the metabolism of glycated proteins, but does not show sialoglycoprotease activity against glycophorin A.
Gene References into Functions
  1. This study showed that the transcription of the thr and ilv operons in ygjD mutants was increased through the inhibition of transcription attenuation and that dnaG transcription was reduced. PMID: 21873492
  2. Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. PMID: 21619589
  3. Escherichia coli containing a conserved Gcp prevents the accumulation of toxic glycated proteins. PMID: 20824107
  4. Repression of ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli. PMID: 19376873
Subcellular Location
Cytoplasm.
Protein Families
KAE1 / TsaD family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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