Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TT16 Polyclonal antibody

Uniprot No.

Q8RYD9

Target Names

TT16

Alternative Names

TT16 antibody; ABS antibody; AGL32 antibody; At5g23260 antibody; MKD15.12 antibody; Protein TRANSPARENT TESTA 16 antibody; Arabidopsis BSISTER MADS-box protein antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana TT16 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor involved in the developmental regulation of the endothelium and in the accumulation of proanthocyanidins (PAs) or condensed tannins which give the seed its brown pigmentation after oxidation. Necessary for the normal activation of the BANYULS promoter in the endothelium body. Is required, together with AGL11/STK for the maternal control of endothelium formation, which is essential for female gametophyte development and fertilization, and seed formation. Interacts genetically with AGL1/SHP1 and AGL5/SHP2 in a partially antagonistic manner and represses AGL1/SHP1, AGL5/SHP2, and AGL8/FUL during flower development. Is essential for the coordination of cell divisions in ovule, seed coat development and endosperm formation. Mediates the crosstalk between endothelium and nucellus to ensure proper seed formation. Functions redundantly with AGL63/GOA to repress nucellus growth and promote its degeneration. Represses the negative regulator of autophagy and programmed cell death HVA22D in the proximal nucellus. Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3'.

Gene References into Functions

the roles and mode of action of TT16 and TT15 during seed development and proanthocyanidin biosynthesis PMID: 28830101
Study suggests that ABS is not only required for the regulation of PA accumulation in the seed coat but positively regulates coordinated cell divisions in the inner integument layer of the ovule, nuclear endosperm proliferation, endothelium cell morphology, and influences on mucilage release of the seed coat epidermal cell layer. PMID: 27776173
No shared protein interaction partners for ABS and GOA could be identified. PMID: 20598091

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in buds, flowers and immature seeds, but not in roots, stems, leaves, seedlings or siliques valves. Expression in seed coat is confined to the endothelium layer.

Database Links

KEGG: ath:AT5G23260

STRING: 3702.AT5G23260.2

UniGene: At.31019

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA848274XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

TT16 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background