Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (Baker's yeast) TY1B Polyclonal antibody

Uniprot No.

Q07163

Target Names

TY1B

Alternative Names

TY1B antibody; POL antibody; TYB1 antibody; Transposon TyH3 Gag-Pol polyprotein antibody; Gag-Pol-p199 antibody; TY1A-TY1B antibody; Transposon Ty1-H3 TYA-TYB polyprotein antibody; p190) [Cleaved into: Capsid protein antibody; CA antibody; Gag-p45 antibody; p54); Ty1 protease antibody; PR antibody; EC 3.4.23.- antibody; Pol-p20 antibody; p23); Integrase antibody; IN antibody; Pol-p71 antibody; p84 antibody; p90); Reverse transcriptase/ribonuclease H antibody; RT antibody; RT-RH antibody; EC 2.7.7.49 antibody; EC 2.7.7.7 antibody; EC 3.1.26.4 antibody; Pol-p63 antibody; p60)] antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (Baker's yeast) TY1B protein

Immunogen Species

Saccharomyces cerevisiae (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty1 RNA and initiation of reverse transcription.; The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.; Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.; Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.

Subcellular Location

Cytoplasm. Nucleus.

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TY1B Antibody

Product Details

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Target Background