U2af1l4 Antibody

Code CSB-PA805251XA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) U2af1l4 Polyclonal antibody
Uniprot No.
Target Names
U2af1l4
Alternative Names
U2af1l4 antibody; U2af26 antibody; Splicing factor U2AF 26 kDa subunit antibody; U2 auxiliary factor 26 antibody; U2 small nuclear RNA auxiliary factor 1-like protein 4 antibody; U2AF1-like 4 antibody
Raised in
Rabbit
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) U2af1l4 protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. It can functionally substitute for U2AF1 in constitutive splicing and enhancer-dependent splicing. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site. Shows a preference for AGC or AGA. Alternative splicing of U2AF1L4 may play a role in connecting the circadian rhythm to changing external cues: may provide a circadian buffering system in central and periphery clocks that allows synchronized adaption to clock-resetting stimuli in order to prevent potentially pathogenic desynchronization.
Gene References into Functions
  1. Data suggest light-induced U2af26 alternative splicing to be a buffering mechanism that limits PERIOD1 induction, thus stabilizing the circadian clock against abnormal changes in light:dark conditions. PMID: 24837677
  2. Differential isoform expression and interaction with the P32 regulatory protein controls the subcellular localization of the splicing factor U2AF26 PMID: 18460468
Subcellular Location
[Isoform 1]: Nucleus. Nucleus speckle. Cytoplasm.; [Isoform 3]: Cytoplasm.; [Isoform 2]: Cytoplasm.
Protein Families
Splicing factor SR family
Tissue Specificity
Ubiquitous. Highly expressed in the brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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