UBA4 Antibody

Code CSB-PA413848XA01STA
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) UBA4 Polyclonal antibody
Uniprot No.
Target Names
UBA4
Alternative Names
UBA4 antibody; NCS3 antibody; SCY_2503Adenylyltransferase and sulfurtransferase UBA4 antibody; Needs CLA4 to survive protein 3 antibody; Ubiquitin-like protein activator 4) [Includes: Adenylyltransferase UBA4 antibody; EC 2.7.7.-); Sulfurtransferase UBA4 antibody; EC 2.8.1.-)] antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) UBA4 protein
Immunogen Species
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Subcellular Location
Cytoplasm.
Protein Families
HesA/MoeB/ThiF family, UBA4 subfamily
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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