UbcE2M Antibody

Code CSB-PA894731XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) UbcE2M Polyclonal antibody
Uniprot No.
Target Names
UbcE2M
Alternative Names
UbcE2M antibody; Ubc12 antibody; CG7375 antibody; Nedd8-conjugating enzyme UbcE2M antibody; EC 2.3.2.- antibody; Nedd8 carrier protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) UbcE2M protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Accepts the ubiquitin-like protein Nedd8 from the Uba3-APP-BP1 E1 complex and catalyzes its covalent attachment to other proteins. Required for Cul1 and Cul3 neddylation. Negatively regulates full-length ci stability and hedgehog signaling.
Gene References into Functions
  1. Data show that two novel genes dUba3 (CG13343) and dUbc12 ( CG7375) played a critical role in Hh signal transduction. PMID: 21931660
Protein Families
Ubiquitin-conjugating enzyme family, UBC12 subfamily
Tissue Specificity
Expressed in the wing disk.
Database Links

KEGG: dme:Dmel_CG7375

STRING: 7227.FBpp0304449

UniGene: Dm.11643

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