Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD6 Polyclonal antibody

Uniprot No.

P06104

Target Names

RAD6

Alternative Names

RAD6 antibody; UBC2 antibody; YGL058W antibody; Ubiquitin-conjugating enzyme E2 2 antibody; EC 2.3.2.23 antibody; E2 ubiquitin-conjugating enzyme 2 antibody; Radiation sensitivity protein 6 antibody; Ubiquitin carrier protein UBC2 antibody; Ubiquitin-protein ligase UBC2 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAD6 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. In association with the E3 enzyme BRE1 and LGE1, it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In association with the E3 enzyme RAD18, it catalyzes the monoubiquitination of POL30 'Lys-164', involved in postreplication repair of UV-damaged DNA. The RAD6/UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine. In association with the E3 enzyme UBR1, is involved in N-end rule-dependent protein degradation. Also involved in sporulation.

Gene References into Functions

Free ubiquitin interacts weakly with both non-canonical and canonical backside residues of Rad6 and that mutations of non-canonical residues have deleterious effects on Rad6 activity. PMID: 26286193
Rad6 boosts the activity of the cell cycle machinery. [Review] PMID: 22214660
These data indicate that the RAD6 gene is involved in the HS-induced BLM resistance observed in the isogenic wild-type strain. PMID: 15605356
RAD6 and its epistasis group members have roles in spontaneous polzeta-dependent translesion synthesis in Saccharomyces cerevisiae PMID: 15687278
Asf1 and Rad6 cooperate in ubiquitination of proliferating cell nuclear antigen (PCNA), indicating that Rad6 and Asf1 function in parallel pathways that ubiquitinate PCNA PMID: 19635810

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Ubiquitin-conjugating enzyme family

Database Links

KEGG: sce:YGL058W

STRING: 4932.YGL058W

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RAD6 Antibody

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Target Background